Article first published online: 04 Jun 2004
Background Captopril is an angiotensin‐converting enzyme inhibitor with sulphydryl groups in its chemical structure. It is commonly used as an antihypertensive drug. The occurrence of pemphigus vulgaris has repeatedly been reported in patients receiving captopril. The capacity of captopril and pemphigus serum to induce acantholysis, in vivo or in vitro, has been demonstrated experimentally.
Objectives To show that captopril and pemphigus serum, acting by a biochemical and immunological mechanism, respectively, trigger apoptosis.
Methods Human keratinocyte cells were treated with 15 mmol L−1 captopril or with pemphigus serum. DNA was extracted and the terminal deoxynucleotidyl transferase‐mediated deoxyuridine triphosphate nick end labelling method was used to detect apoptosis.
Results DNA fragmentation occurred after 72 h of treatment. Increased expression of p53, c‐myc and inducible nitric oxide (NO) synthase (iNOS) mRNA were observed by polymerase chain reaction (PCR) in the treated cells compared with the untreated ones. The increase in iNOS gene expression was associated with overproduction of NO. Moreover, the addition of 1 mmol L−1N‐monomethyl‐
Conclusions These data demonstrate the involvement of apoptosis in keratinocytes treated with captopril or pemphigus serum, with induction of the iNOS gene and hsp70 in the cascade of events leading to programmed cell death.Read moreRead more (PDF)
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